E-ISSN 2146-8311
 

Original Research 


Occurrence and the level of contamination of aflatoxin M1in raw, pasteurized, and UHT buffalo milk consumed in Sohag and Assiut, Upper Egypt

Eman M Shaker, Eman E Elsharkawy.

Cited by (5)

Abstract
Aim: This study evaluated the aflatoxin M1 (AFM1) in raw, pasteurized and ultra-heat treated (UHT) buffalo milk consumed in upper Egypt. Materials and Methods: Milk samples were collected from January to June 2013. The competitive enzyme-linked immunosorbent assay was applied. Results: All the tested samples were positive for AFM1, which were above the Egyptian regulations limits 0 ng/L. The raw milk showed 93% of samples were above the permitted limit set by the European Commission (EC), whereas 3.3% of samples exceeded US Food and Drug Administration (US FDA) tolerance limit. The pasteurized milk showed 100% and 13.5% of samples exceeded the EC, and US FDA, respectively. UHT milk Brand I and II showed 100% and 86.5% of samples were above EC, and 36.6% and 6.6% of samples exceeded US FDA, respectively. Conclusion: These results raise concerns about the milk consumed in Egypt and it must be monitored for AFM1 contamination.

Key words: Aflatoxin M1, buffalo milk, enzyme-linked immunosorbent assay


 
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REFERENCES
1. IARC. Some Naturally Occurring Substances: Food Items andConstituents, Hetero-Cyclic Aromatic Amines and Mycotoxins - IARCMonographs on the Evaluation of Carcinogenic Risks to Humans.Lyon: International Agency for Research on Cancer; 1993.
2. Reddy KR, Farhana NI, Salleh B. Occurrence of Aspergillus spp. andaflatoxin B1 in Malaysian foods used for human consumption. J FoodSci 2011;76:T99-104. [DOI via Crossref]    [Pubmed]   
3. Battacone G, Nudda A, Cannas A, Cappio Borlino A, Bomboi G,Pulina G. Excretion of aflatoxin M1 in milk of dairy ewes treated withdifferent doses of aflatoxin B1. J Dairy Sci 2003;86:2667-75. [DOI via Crossref]   
4. Galvano F, Galofaro V, Galvano G. Occurrence and stability of aflatoxinM1 in milk and milk products: A worldwide review. J Food Prot1996;59:1079-90.
5. Tekinsen KK, Eken HS. Aflatoxin M1 levels in UHT milk and kasharcheese consumed in Turkey. Food Chem Toxicol 2008;46:3287-9. [DOI via Crossref]    [Pubmed]   
6. JECFA. Safety evaluation of certain mycotoxins in food. In Prepared bythe 56th Meeting of the Food Additives Series No. 47, 2001. Availablefrom: http://www.inchem.org/documents/jecfa/jecmono/v47je02.htm. [Last retrieved on 2011 Jan 04].
7. Roussi V, Govaris A, Varagouli A, Botsoglou NA. Occurrence ofaflatoxin M(1) in raw and market milk commercialized in Greece.Food Addit Contam 2002;19:863-8. [DOI via Crossref]    [Pubmed]   
8. Stoloff L, Van Egmond HP, Park DL. Rationales for the establishmentof limits and regulations for mycotoxins. Food Addit Contam1991;8:213-21. [DOI via Crossref]    [Pubmed]   
9. EC. Commission regulation 1881/2006 of 19 December settingmaximum levels for certain contaminants in foodstuffs. Official JEur Union 2006;L364:5-24.
10. CAC. Commission submitted on the draft maximum level for aflatoxinM1 in milk. Hague, Netherlands: Codex Committee on Food Additivesand Contamination 33rd Session; 2001.
11. FAO. Worldwide Regulations for Mycotoxins in Food and Feed in2003. FAO Food and Nutrition Paper 81. Rome: Food and AgricultureOrganization of the United Nations; 2004.
12. Rahimi E, Bonyadian M, Rafei M, Kazemeini HR. Occurrence ofaflatoxin M1 in raw milk of five dairy species in Ahvaz, Iran. FoodChem Toxicol 2010;48:129-31. [DOI via Crossref]    [Pubmed]   
13. FDA. Sec. 527.400 whole milk, low fat milk, skim milk and aflatoxinM1 (CPG7106.10). FDA/ORA Compliance Policy Guides; 2005.
14. EFSA. Opinion of the scientific panel on contaminants in the foodchain on a request from the commission related to aflatoxin B1 asundesirable substance in animal feed. EFSA J 2004;39:1-27.
15. EC. Commission regulation 683/2004 of 13 April 2004 amendingregulation No. 466/2001 of 8 March 2001 as regards aflatoxins andochratoxin a in foods for infants and young children. Official J EurCommun 2004;L106:3-5.
16. EC. Commission Directive 2003/100/EC of 31 October 2003 amendingannex I to directive 2002/32/EC of the European Parliament and ofthe Council on undesirable substances in animal feed. Official J EurUnion 2003;L285:33-7.
17. Anfossi L, Baggiani C, Giovannoli C, Giraudi G. Occurrenceof aflatoxin M1 in dairy products. In: Torres-Pacheco I, editor.Aflatoxins-Detection, Measurement and Control. USA: InTech; 2011.ISBN: 978-953-307-711-6. Available from: http://www.intechopen.com/books/aflatoxins-detectionmeasurement-and-control/occurrence-of-aflatoxin-m1-in-dairy-products.
18. Egyptian Regulations. Maximum Limits for Mycotoxin in Foods. Part LAflatoxins E.S. 1875-1990. Egyptian Organization for Standardizationand Quality Control; 1990.
19. Amer AA, Ibrahim MA. Determination of aflatoxin M1 in raw milk andtraditional cheeses retailed in Egyptian markets. J Toxicol EnvironHealth Sci 2010;2:50-3.
20. FDA U.S. Guidance for Industry: Action Levels for Poisonous orDeleterious Substances in Human Food and Animal Feed. Food andDrug Administration; (April 2011) 20/04/2011. Available from: http://www.fda.gov/Food/GuidanceComplianceRegulatoryInformation/GuidanceDocuments/ChemicalContaminantsandPesticides/ucm077969.htm.
21. Salem DA. Natural occurrence of aflatoxin in feedstuffs and milkof dairy farm in Assuit province, Egypt. Wien Tierarztl Monatsschr2002;89:86-91.
22. Motawee MM, Meyer M, Bauer J. Incidence of aflatoxin M1 and B1in raw milk and some dairy products in Damietta. Egypt J Agric SciMansoura Univ 2004;29:711-8.
23. Motawee MM, Meyer M, Bauer J. Occurrence of aflatoxin M1 andB1 in milk and some milk products in Mansoura. Egypt J Agric SciMansoura Univ 2004;29:719-25.
24. Motawee MM, Bauer J, McMahon DJ. Survey of aflatoxin M(1) incow, goat, buffalo and camel milks in Ismailia-Egypt. Bull EnvironContam Toxicol 2009;83:766-9. [DOI via Crossref]    [Pubmed]   
25. Ghanem I, Orfi M. Aflatoxin M1 in raw, pasteurized and powderedmilk available in the Syrian market. Food Control 2009;20:603-5. [DOI via Crossref]   
26. Zinedine A, González-Osnaya L, Soriano JM, Moltó JC, Idrissi L,Ma-es J. Presence of aflatoxin M1 in pasteurized milk from Morocco.Int J Food Microbiol 2007;114:25-9. [DOI via Crossref]    [Pubmed]   
27. Unusan N. Occurrence of aflatoxin M1 in UHT milk in Turkey. FoodChem Toxicol 2006;44:1897-900. [DOI via Crossref]    [Pubmed]   
28. Razza R. Occurrence of aflatoxin M1 in the milk marketed in the cityof Karachi, Pakistan. J Chem Soc 2006;28:155-7.
29. Oliveira CF, Soares NF, Oliveira TV, Júnior JC, Silva WA. Aflatoxin M1occurrence in ultrahigh temperature (UHT) treated fluid milk fromMinas Gerais/Brazil. Food Control 2013;30:90-2. [DOI via Crossref]   
30. Gomaa GM, Deeb AM. Occurrence of aflatoxin M1 in milk collectedfrom Kafr El- Sheikh, Egypt. Zag Vet J 2010;38:144-50.
31. Dashti B, Al-Hamli S, Alomirah H, Al-Zenki S, Abbas AB, Sawaya W.Levels of aflatoxin M1 in milk, cheese consumed in Kuwait andoccurrence of total aflatoxin in local and imported animal feed. FoodControl 2009;20:686-90. [DOI via Crossref]   
32. Buket Er, Demirhan B, Onurdag FK, Yentur G. Determination ofaflatoxin M1 in milk and white cheese consumed in Ankara region,Turkey. J Anim Vet Adv 2010;9:1780-4. [DOI via Crossref]   
33. Ardic M, Karakaya Y, Atasever M, Adiguzel G. Aflatoxin M1 levels ofTurkish white brined cheese. Food Control 2009;l20:196-9.
34. Oliveira CA, Ferraz JC. Occurrence of aflatoxin M1 in pasteurized, UHTmilk and milk powder from goat origin. Food Control 2007;18:375-8. [DOI via Crossref]   
35. Zheng N, Sun P, Wang JQ, Zhen YP, Han RW, Xu XM. Occurrence ofaflatoxin M1 in UHT milk and pasteurized milk in China market. FoodControl 2013;29:198-201. [DOI via Crossref]   
36. Bilandzic N, Varenina I, Solomun B. Aflatoxin M1 in raw milk in Croatia.Food Control 2010;21:1279-81. [DOI via Crossref]   
37. Prandini A, Tansini G, Sigolo S, Filippi L, Laporta M, Piva G. On theoccurrence of aflatoxin M1 in milk and dairy products. Food ChemToxicol 2009;47:984-91. [DOI via Crossref]    [Pubmed]   
38. Tajkarimi M, Shojaee Aliabadi F, Salah Nejad M, Pursoltani H,Motallebi AA, Mahdavi H. Seasonal study of aflatoxin M1 contaminationin milk in five regions in Iran. Int J Food Microbiol 2007;116:346-9. [DOI via Crossref]    [Pubmed]   

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How to Cite this Article
Pubmed Style

Eman M Shaker, Eman E Elsharkawy. Occurrence and the level of contamination of aflatoxin M1in raw, pasteurized, and UHT buffalo milk consumed in Sohag and Assiut, Upper Egypt. J Environ Occup Sci. 2014; 3(3): 136-140. doi:10.5455/jeos.20140619064326


Web Style

Eman M Shaker, Eman E Elsharkawy. Occurrence and the level of contamination of aflatoxin M1in raw, pasteurized, and UHT buffalo milk consumed in Sohag and Assiut, Upper Egypt. http://www.jeosci.com/?mno=158758 [Access: October 15, 2019]. doi:10.5455/jeos.20140619064326


AMA (American Medical Association) Style

Eman M Shaker, Eman E Elsharkawy. Occurrence and the level of contamination of aflatoxin M1in raw, pasteurized, and UHT buffalo milk consumed in Sohag and Assiut, Upper Egypt. J Environ Occup Sci. 2014; 3(3): 136-140. doi:10.5455/jeos.20140619064326



Vancouver/ICMJE Style

Eman M Shaker, Eman E Elsharkawy. Occurrence and the level of contamination of aflatoxin M1in raw, pasteurized, and UHT buffalo milk consumed in Sohag and Assiut, Upper Egypt. J Environ Occup Sci. (2014), [cited October 15, 2019]; 3(3): 136-140. doi:10.5455/jeos.20140619064326



Harvard Style

Eman M Shaker, Eman E Elsharkawy (2014) Occurrence and the level of contamination of aflatoxin M1in raw, pasteurized, and UHT buffalo milk consumed in Sohag and Assiut, Upper Egypt. J Environ Occup Sci, 3 (3), 136-140. doi:10.5455/jeos.20140619064326



Turabian Style

Eman M Shaker, Eman E Elsharkawy. 2014. Occurrence and the level of contamination of aflatoxin M1in raw, pasteurized, and UHT buffalo milk consumed in Sohag and Assiut, Upper Egypt. Journal of Environmental and Occupational Science, 3 (3), 136-140. doi:10.5455/jeos.20140619064326



Chicago Style

Eman M Shaker, Eman E Elsharkawy. "Occurrence and the level of contamination of aflatoxin M1in raw, pasteurized, and UHT buffalo milk consumed in Sohag and Assiut, Upper Egypt." Journal of Environmental and Occupational Science 3 (2014), 136-140. doi:10.5455/jeos.20140619064326



MLA (The Modern Language Association) Style

Eman M Shaker, Eman E Elsharkawy. "Occurrence and the level of contamination of aflatoxin M1in raw, pasteurized, and UHT buffalo milk consumed in Sohag and Assiut, Upper Egypt." Journal of Environmental and Occupational Science 3.3 (2014), 136-140. Print. doi:10.5455/jeos.20140619064326



APA (American Psychological Association) Style

Eman M Shaker, Eman E Elsharkawy (2014) Occurrence and the level of contamination of aflatoxin M1in raw, pasteurized, and UHT buffalo milk consumed in Sohag and Assiut, Upper Egypt. Journal of Environmental and Occupational Science, 3 (3), 136-140. doi:10.5455/jeos.20140619064326





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